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primary human small airway epithelial cells hsaecs  (ATCC)
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ATCC primary human small airway epithelial cells hsaecs
Primary Human Small Airway Epithelial Cells Hsaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hsaecs  (ATCC)
98
ATCC hsaecs
Hsaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hsaecs/product/ATCC
Average 98 stars, based on 1 article reviews
hsaecs - by Bioz Stars, 2026-02
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primary hsaec  (ATCC)
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ATCC primary hsaec
Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small <t>airway</t> <t>epithelial</t> cells <t>(HSAEC)</t> treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).
Primary Hsaec, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary hsaec/product/ATCC
Average 98 stars, based on 1 article reviews
primary hsaec - by Bioz Stars, 2026-02
98/100 stars
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primary small airway epithelial cells hsaecs  (ATCC)
97
ATCC primary small airway epithelial cells hsaecs
Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small <t>airway</t> <t>epithelial</t> cells <t>(HSAEC)</t> treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).
Primary Small Airway Epithelial Cells Hsaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary small airway epithelial cells hsaecs/product/ATCC
Average 97 stars, based on 1 article reviews
primary small airway epithelial cells hsaecs - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
hsaec  (ATCC)
98
ATCC hsaec
Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small <t>airway</t> <t>epithelial</t> cells <t>(HSAEC)</t> treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).
Hsaec, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hsaec/product/ATCC
Average 98 stars, based on 1 article reviews
hsaec - by Bioz Stars, 2026-02
98/100 stars
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primary hsaecs  (ATCC)
98
ATCC primary hsaecs
Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small <t>airway</t> <t>epithelial</t> cells <t>(HSAEC)</t> treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).
Primary Hsaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary hsaecs/product/ATCC
Average 98 stars, based on 1 article reviews
primary hsaecs - by Bioz Stars, 2026-02
98/100 stars
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Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small airway epithelial cells (HSAEC) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).

Journal: Physiological Reports

Article Title: Secreted mitochondrial aspartyl‐ tRNA synthetase ( DARS2 ) regulates TNFα signaling

doi: 10.14814/phy2.70627

Figure Lengend Snippet: Secreted DARS2 from airway epithelia binds to macrophages. (a) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (b) quantitation of DARS2 in the supernatant from primary human small airway epithelial cells (HSAEC) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (c) Immunoreactive levels of DARS2 in the cell lysate (top) and supernatant (bottom) and (d) quantitation of DARS2 in the supernatant from human alveolar macrophages (HAM) treated with Pam, IL‐1β or increasing concentrations of TNFα (repeated for n = 3 representative blots shown). (e) Schematic of adoptive media transfer from BEAS‐2Bs to recipient PMA‐stimulated THP1‐macrophages. BEAS2Bs were transfected with FLAG‐tagged empty vector (EV) or DARS2‐Flag plasmid and media was transferred to THP‐1 cells. (f) Shown are levels of ectopically expressed DARS2 in THP1 cell lysates (each lane is a biologically independent replicate, repeated for n = 2). (g) Immunofluorescence of THP1‐macrophages receiving media from donor BEAS‐2B cells transfected with DARS2‐FLAG as in (e, f) showing co‐localization of DARS2‐Flag with the actin filament marker, phalloidin (arrows, merge panels) Scale bar = 20 μm (repeated for n = 2). (h) IL18 , (i) TNF , (j) CXCL1 mRNA levels by RT‐qPCR analysis in THP1‐macrophages 24 h after receiving media from donor BEAS‐2B cells transfected with EV‐FLAG or DARS2‐FLAG ( n = 8 biological replicates/group).

Article Snippet: BEAS‐2B (Human Bronchial Epithelial cells, ATCC, catalog no. CRL‐35588), THP‐1 (Tohoku Hospital Pediatrics‐1, an acute myeloid leukemia line used as a model of monocytes, ATCC, catalog no. TIB‐202), primary HSAEC (human small airway epithelial cells, ATCC, catalog no. PCS‐301‐010), and primary HBEC (human bronchial epithelial cells, ATCC, catalog no. PCS‐300‐010) were used for this study.

Techniques: Quantitation Assay, Transfection, Plasmid Preparation, Immunofluorescence, Marker, Quantitative RT-PCR